《植物生理学报》 2017, 53(1): 126-132

超声波协同酶法优化芦蒿多糖提取工艺

吴雨龙^1,2, 江海涛¹, 汪振炯¹, 王仁雷³, 华春^1,*, 周峰^1,2,*

¹南京晓庄学院食品科学学院, 南京211171; ²山东省黄河三角洲生态环境重点实验室, 山东滨州256603; ³江苏第二师范学院生命科学与化学化工学院, 南京210013

通信作者：华春；E-mail: hc3501988@163.com; zfibcas@163.com

摘　要：

为了优化超声波协同酶法提取芦蒿多糖的工艺条件, 采用中心组合设计方法(Box-Behnken design), 建立以超声时间、超声温度、加酶量、酶解时间和液料比对芦蒿多糖得率的影响的二次回归模型。获得超声波协同酶法提取芦蒿多糖的最佳工艺条件为超声时间40 min、超声温度50°C、加酶量1.5%、酶解时间1 h、液料比40:1 (mL·g^-1), 在此条件下, 芦蒿多糖的得率为37.19%。结果表明该回归模型有极显著性, 可以作为芦蒿多糖提取工艺的回归分析和参数优化。

关键词：芦蒿; 多糖; 超声波法; 纤维素酶; 中心组合设计方法

收稿：2016-08-11   修定：2016-11-15

资助：国家高技术研究发展计划(2012AA021701)、国家自然科学基金(21376112)、江苏省自然科学基金(BK20141081)、山东省黄河三角洲生态环境重点实验室开放基金(2015-KFJJ04)和南京市环境科学与工程重点学科建设项目。

Optimization of extraction technology of polysaccharide from Artemisia selengensis Turcz. by ultrasonic assisted enzymatic method

WU Yu-Long^1,2, JIANG Hai-Tao¹, WANG Zhen-Jiong¹, WANG Ren-Lei³, HUA Chun^1,*, ZHOU Feng^1,2,*

¹School of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China; ²Shandong Provincial Key Laboratory of Eco-environmental Science for Yellow River Delta, Binzhou, Shandong 256603, China; ³School of Life Science and Chemistry, Jiangsu Second Normal University, Nanjing 210013, China

Corresponding author: HUA Chun; E-mail: hc3501988@163.com; zfibcas@163.com

Abstract:

In order to optimize the extraction technology of polysaccharide from Artemisia selengensis Turcz. by ultrasonic assisted enzymatic method, the Box-Behnken design was used to establish the effects of ultrasonic time, ultrasonic temperature, enzyme dosage, enzyme solution time and liquid to solid ratio on Artemisia polysaccharide yield of quadratic regression model. The optimum extraction conditions of ultrasonic assisted enzymatic method extraction of polysaccharide from Artemisia selengensis Turcz. were ultrasonic time 40 min, ultrasonic temperature 50°C, enzyme dosage 1.5%, enzymolysis time 1 h, liquid-to-solid ratio of 40:1 (mL·g^-1), under these conditions, the extraction of Artemisia polysaccharide was 37.19%. The results showed that the regression model was significant, which could be used as the regression analysis and parameter optimization of the extraction technology of Artemisia polysaccharide.

Key words: Artemisia selengensis Turcz.; polysaccharide; ultrasonic method; cellulose; Box-Behnken design